Probe JC-1 staining was used to measure the mitochondrial membrane potential in renal tubule epithelial cells. Post-treatment, medium/JC-1 working solution (1:1) was added to the cell slides in the plate and incubated for 20 min. The staining solution was removed, and then the cells were gently washed twice with JC-1 staining buffer. The pictures were captured by a Ti2-A fluorescence microscope (NIKON, Tokyo, Japan) [43 (link)].
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