Example 6
This example provides a representative example of an aqueous solution to be used in a method described herein. The composition may contain the following ingredients:
The aqueous solution is formulated in physiological saline and adjusted to about pH 7.4, thereby minimizing any injecting pain beyond the needle prick. In addition, the aqueous solution may be optionally supplemented with a preservative (e.g., benzyl alcohol), a chemical stabilizer (e.g., gentisic acid), and/or an additional bioactive agent (e.g., platelet-rich plasma) depending on anticipated delivery method, shelf-life, and intended effects.
Example 7
The purpose of this example is to evaluate the efficacy of a composition described herein in treating the signs of aging present on facial skin. A composition is prepared as in Example 6.
Two groups of human subjects (8-10 subjects per group) are injected with the composition or physiological saline (control) twice a day for a period of 29 days. At the end of the 29 day test period, the subjects are polled regarding various aspects of the effectiveness of the composition described herein in treating and/or minimizing signs of aging present on the skin. The following aspects of the composition described herein are evaluated:
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- (1) effectiveness of the composition described herein in improving the smoothness and/or softness of skin (i.e., making the skin feel smoother and softer following treatment);
- (2) effectiveness of the composition described herein in improving the overall appearance of skin;
- (3) effectiveness of the composition described herein in evening out skin tone and texture;
- (4) effectiveness of the composition described herein in improving the clarity and/or radiance of skin;
- (5) effectiveness of the composition described herein in making the skin look younger; and
- (6) effectiveness of the composition described herein in making wrinkles appear softer and/or less prominent.
- (7) effectiveness of the composition described herein in increasing the degree of hydration of the skin.
Patients treated with the composition exhibit improvement in one or more of the symptoms described herein.
Example 8
The purpose of this example is to evaluate the efficacy of a composition described herein in treating the signs of aging present on facial skin. A composition is prepared as in Example 6.
Two groups of human subjects (8-10 subjects per group) are injected with the composition or physiological saline (control) on days 1, 3, 7, 10, 14, 21, 30, 60, and 90 of treatment. At the end of the 90-day test period, the subjects are polled regarding various aspects of the effectiveness of the composition described herein in treating and/or minimizing signs of aging present on the skin. The following aspects of the composition described herein are evaluated:
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- (1) effectiveness of the composition described herein in improving the smoothness and/or softness of skin (i.e., making the skin feel smoother and softer following treatment);
- (2) effectiveness of the composition described herein in improving the overall appearance of skin;
- (3) effectiveness of the composition described herein in evening out skin tone and texture;
- (4) effectiveness of the composition described herein in improving the clarity and/or radiance of skin;
- (5) effectiveness of the composition described herein in making the skin look younger; and
- (6) effectiveness of the composition described herein in making wrinkles appear softer and/or less prominent.
- (7) effectiveness of the composition described herein in increasing the degree of hydration of the skin.
Patients treated with the composition exhibit improvement in one or more of the symptoms described herein.
While preferred embodiments have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the embodiments. It should be understood that various alternatives to the embodiments described herein may be employed. It is intended that the following claims define the scope of the embodiments and that methods and structures within the scope of these claims and their equivalents be covered thereby.
