Rats were injected with meloxicam 2 mg/kg s.c. (Metacam; Boehringer Ingelheim Vetmedica, St. Joseph, MO), anesthetized with 2.5% isoflurane in oxygen, and the incision site infiltrated with 0.25% bupivacaine (Marcaine; Hospira, Lake Forest, IL). Adrenal glands were then located through bilateral incisions in the abdominal wall, their capsules incised, and the medullae excised3 (link),44 (link),62 (link); the fascia and skin were then sutured separately. Rats were provided with 0.45% saline to drink (ad libitum) for the first 7 days after surgery and received an additional injection of meloxicam 24 hours after surgery. To allow for maximum recovery of hypothalamic–pituitary–adrenal axis function, adrenal medullectomy was performed at least 5 weeks before additional experimental procedures.62 (link)
Protocol detail
Adrenal Medullectomy in Rats
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Abdominal wall
Adrenal glands
Bupivacaine
Capsules
Fascia
Forest
Hypothalamic
Isoflurane
Marcaine
Medullae
Meloxicam
Oxygen
Pituitary adrenal axis
Rats
Recovery of function
Saline
Skin
Surgery
Corresponding Organization : University of California, San Francisco
Top 5 similar protocols
Variable analysis
independent variables
- Meloxicam (2 mg/kg s.c.)
- Isoflurane (2.5% in oxygen)
- Bupivacaine (0.25%)
- Adrenal medullectomy
- Hypothalamic–pituitary–adrenal axis function
- Saline (0.45% to drink ad libitum for the first 7 days after surgery)
- Additional injection of meloxicam 24 hours after surgery
- Adrenal medullectomy performed at least 5 weeks before additional experimental procedures
- Positive controls not explicitly mentioned
- Negative controls not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!