TEM Analysis of oEV Integrity and Morphology

The analysis of the oEV integrity and morphology was performed using transmission electron microscopy (TEM), as previously described [19 (link)]. Briefly, oEVs were left to adhere for 20 min on 200 mesh nickel formvar carbon-coated grids (Electron Microscopy Science, Hatfield, PA, USA). Subsequently, grids were incubated with 2.5% glutaraldehyde plus 2% sucrose and washed with distilled water. Finally, samples were negatively stained with Nano-Van and Nano-W (Nanoprobes, Yaphank, NY, USA) and acquired with Jeol JEM 1400 Flash electron microscope (Jeol, Tokyo, Japan). The analysis was repeated in three independent experiments.

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Pomatto M.A., Gai C., Negro F., Massari L., Deregibus M.C., De Rosa F.G, & Camussi G. (2023). Oral Delivery of mRNA Vaccine by Plant-Derived Extracellular Vesicle Carriers. Cells, 12(14), 1826.

Publication 2023

nickel formvar carbon-coated grids Nano-Van Nano-W JEM 1400 Flash electron microscope

Carbon Formvar Glutaraldehyde Microscope electron Nickel Sucrose Transmission electron microscopy

Corresponding Organization : University of Turin

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Variable analysis

independent variables

Incubation time of oEVs on grids (20 min)

dependent variables

OEV integrity and morphology

control variables

Formvar carbon-coated grids
Glutaraldehyde and sucrose fixation
Negative staining with Nano-Van and Nano-W
Electron microscope (Jeol JEM 1400 Flash)

controls

Positive control: Not mentioned
Negative control: Not mentioned

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