Cultured cell phenotypes were analyzed through direct immune fluorescence staining of cell surface antigens using fluorescein isothiocyanate (FITC), peridinin-chlorophyll-protein (PerCP), phycoerythrin (PE), or Allophycocyanin (APC) conjugated antibodies against MHC I/II, CD11c, CD80/86 and CD14 (eBioscience) by flow cytometry. Fluorescence-labeled IgG isotypes were selected as the control. All antibodies were used at manufacturer-recommended concentrations.
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Phenotypic Analysis of Cultured Cells
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Other organizations : Chung-Ang University Hospital, Ulsan College, Asan Medical Center, University of Ulsan, Ewha Womans University
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Variable analysis
independent variables
- Cell surface antigen staining with FITC, PerCP, PE, or APC conjugated antibodies against MHC I/II, CD11c, CD80/86 and CD14
- Cultured cell phenotypes
- Fluorescence-labeled IgG isotypes as control
- Fluorescence-labeled IgG isotypes were selected as the control
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