This was a retrospective study of 41 HIV-infected individuals from the HIV Neurobehavioral Research Center (HNRC)’s Prospective Memory cohort [11 (link)]. At the time of sampling, all participants were on ART with undetectable levels of HIV RNA in blood (<50 copies/μL of plasma; Amplicor HIV Monitor Test, Roche Molecular Systems Inc.). Blood lymphocyte profiles were obtained by flow-cytometry (CLIA certified local laboratories). Epidemiological, behavioral risk and clinical data were also collected from participants [12 (link)]. Individuals were deemed to have AIDS if they met Centers for Disease Control criteria (http://www.aidsmap.com/CDC-case-definitions/page/1391604/ ). The estimated duration of infection (EDI) was determined using results of serologic and virologic tests as described previously [13 (link)]. Paired stored CSF and blood samples were retrospectively selected and used for measurements of CSF and blood plasma markers of inflammation and cell-free mitochondrial DNA.
Protocol detail
Retrospective Study of HIV-Infected Individuals
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Aids
Blood
Cell free dna
Flow cytometry
Hiv test
Infection
Inflammation of blood
Lymphocyte
Mitochondrial
Plasma
Prospective memory
Corresponding Organization : University of California, San Diego
Other organizations : Western Michigan University, Washington University in St. Louis, University of Houston, VA San Diego Healthcare System
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Variable analysis
independent variables
- None explicitly mentioned
- Markers of inflammation in CSF and blood plasma
- Cell-free mitochondrial DNA in CSF and blood plasma
- All participants were on ART with undetectable levels of HIV RNA in blood (<50 copies/μL of plasma)
- Epidemiological, behavioral risk and clinical data were also collected from participants
- Positive control: Not specified
- Negative control: Not specified
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