Matrigel assay was performed as described previously.49 (link) Briefly, bEnd3 endothelial cells were plated in a six-well Matrigel plate (BD Biocoat Matrigel Matrix, 354432). The cells were seeded at a density of 105 cells per well in 2 mL medium. At 2 hours after seeding in Matrigel, the cells were treated with GSNO (100 μM), 2-ME (5 μM), or GSNO followed by 2-ME (30 minutes later). The cells were observed under a microscope for formation of tube-like structures at 3, 18, and 48 hours. Tubule length ratio, branch number per field, and aggregation area were analyzed using ImageJ software.
Protocol detail
Matrigel Tubulogenesis Assay Protocol
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Medical University of South Carolina
Top 5 similar protocols
Variable analysis
independent variables
- GSNO (100 μM)
- 2-ME (5 μM)
- GSNO followed by 2-ME (30 minutes later)
- Tubule length ratio
- Branch number per field
- Aggregation area
- BEnd3 endothelial cells
- Matrigel plate
- Cell seeding density (10^5 cells per well)
- Medium volume (2 mL)
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!