Histological Processing of SARS-CoV-2 Infected Ferret Turbinates

SARS-CoV-2 VIC01 infection of ferrets is described in Au et al. (2022 (link)). Briefly, ferret turbinates at 7 days post infection were fixed in 10% neutral buffered formalin (Australian Biostain). Preserved ferret turbinates were processed according to routine histological methods, embedded in paraffin wax (Leica Biosystems), and 4-µm serial sections were mounted on glass slides (Menzel Gläser). Tissue sections were deparaffinised using routine histological methods. Antigen retrieval was performed using Target Retrieval Solution, high pH (Dako) at 97°C for 30 min on a PT Link pre-treatment module (Agilent).

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Luczo J.M., Edwards S.J., Ardipradja K., Suen W.W., Au G.G., Marsh G.A., Godde N., Rootes C.L., Bingham J, & Sundaramoorthy V. (2024). SARS-CoV and SARS-CoV-2 display limited neuronal infection and lack the ability to transmit within synaptically connected axons in stem cell–derived human neurons. Journal of Neurovirology, 30(1), 39-51.

Publication 2024

paraffin wax Target Retrieval Solution, high PT Link pre-treatment module

Corresponding Organization :

Other organizations : Australian Centre for Disease Preparedness, Commonwealth Scientific and Industrial Research Organisation, Deakin University

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independent variables

SARS-CoV-2 VIC01 infection of ferrets

dependent variables

Histological analysis of ferret turbinates at 7 days post infection

control variables

Ferret turbinates were fixed in 10% neutral buffered formalin
Ferret turbinates were processed according to routine histological methods
Ferret turbinates were embedded in paraffin wax
4-µm serial sections were mounted on glass slides
Tissue sections were deparaffinised using routine histological methods
Antigen retrieval was performed using Target Retrieval Solution, high pH at 97°C for 30 min on a PT Link pre-treatment module

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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