Renal function was assessed by the eGFR, estimated through the CKD-EPI equation, with one blood sample collection (22 (link)), and by MA, using the albumin-creatinine ratio (ACR) with a 12-h urine collection. We considered abnormal renal function as eGFR<60 mL·min-1·(1.73 m2)-1 and an abnormal MA as ≥30mg/dL (23 (link)). The kinetic Jaffé's method was used to measure urinary creatinine levels (Advia 1200 Siemens, USA), and the immunochemical assay was used to measure urinary albumin (BN II Nephelometer Siemens Dade Behring, USA). All analyses were performed at the University of Sao Paulo.
Free full text: Click here