The MCF-7 cell growth
inhibition assay was carried out as described previously.45 (link) A broth microdilution method was used to determine
the growth inhibition IC50 values. Compounds were half-log
serial diluted using cell culture media into 96-well TC-treated round-bottom
plates (Corning Inc., Corning, NY). Cells were plated at a density
of 5000 cells/well and then incubated under the same culture conditions
for 2 days at which time an MTT ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide) cell proliferation assay (ATCC, Manassas, VA) was performed
to obtain dose–response curves.
inhibition assay was carried out as described previously.45 (link) A broth microdilution method was used to determine
the growth inhibition IC50 values. Compounds were half-log
serial diluted using cell culture media into 96-well TC-treated round-bottom
plates (Corning Inc., Corning, NY). Cells were plated at a density
of 5000 cells/well and then incubated under the same culture conditions
for 2 days at which time an MTT ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide) cell proliferation assay (ATCC, Manassas, VA) was performed
to obtain dose–response curves.
