Super-resolution Imaging of Coke in ZEOL

SIM was applied to gain super-resolution images on the spatiotemporal distribution of the coke species located in ZEOL series samples. The super-resolution imaging was carried out using a Nikon N-SIM super-resolution microscopy system with a motorized inverted microscopy ECLIPSE Ti2-E, a 100×/NA 1.49 oil immersion TIRF objective lens (CFI HP) and ORCA-Flash 4.0 sCMOS camera (Hamamatsu Photonics K.K.)^{61 (link),62 (link)}. Imaging with 405, 488, 561, and 640 nm multilaser light sources, the emissions by excitation were collected with four photomultiplier tubes in the range 435–485, 500–545, 570–640, 663–738 nm for the four lasers, respectively. Imaging was performed under ex-situ condition at room temperature. ZEOL series samples were loaded on glass-bottomed culture dishes (35 mm dish with 20 mm well) and images were taken at a Z-plane of middle of zeolitic crystal. The software NIS-Elements Ar and N-SIM Analysis were used to analyze the collected images and computationally reconstruct the super-resolution image.