Microarray-based Profiling of Germ Cell Cancers

Illumina HT-12v4 expression microarray analysis of GCC cell lines and
PRAME knockdown of cells were performed as described previously
(Nettersheim et al, 2015 (link)). All
microarray data are publically available (ncbi.nlm.nih.gov/geo/)
(GSE71269). Each sample was analysed in three replicates. Affymetrix expression
microarray data of GCC tissues have been re-analysed in context of this study
(Eckert et al, 2008 (link)). In total, 4 normal
testis tissues, 3 GCNIS, 4 seminomas, 3 ECs and 3 teratomas were analysed.

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Nettersheim D., Arndt I., Sharma R., Riesenberg S., Jostes S., Schneider S., Hölzel M., Kristiansen G, & Schorle H. (2016). The cancer/testis-antigen PRAME supports the pluripotency network and represses somatic and germ cell differentiation programs in seminomas. British Journal of Cancer, 115(4), 454-464.

Publication 2016

Cell microarray analysis Cells lines Seminomas Teratomas Tissues

Corresponding Organization :

Other organizations : University of Bonn

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Variable analysis

independent variables

PRAME knockdown of cells

dependent variables

Gene expression profiles of GCC cell lines
Gene expression profiles of GCC tissues

control variables

GCC cell lines without PRAME knockdown
Normal testis tissues
GCNIS (Germ Cell Neoplasia In Situ) tissues
Seminoma tissues
Embryonal carcinoma (EC) tissues
Teratoma tissues

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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