Protein extracts and nuclear extracts were obtained from H9C2 cells and MDA-MB-231 cells. Western blotting was performed as previously described [27 (link),28 (link),29 (link)]. For western blotting, anti-cleaved caspase-3 antibody, anti-cleaved caspase-9 antibody (Abcam, Cambridge, UK), anti-p53 antibody, phospho-p53 (Ser15) antibody (Cell Signaling Technology, Danvers, MA, USA), and anti α-tubulin monoclonal antibody (Fujifilm Wako Pure Chemical Corporation, Osaka, Japan) were used as primary antibodies, and goat anti-rabbit IgG–HRP (MBL, Aichi, Japan) and sheep anti-mouse IgG-HRP (GE Healthcare, Chicago, IL, USA) were used as secondary antibodies.
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