Human tissue microarrays were constructed using the Beecher Instruments Tissue-Arrayer™ (Beecher Instruments); the first consisting of 27 benign, 25 Gleason pattern 3 prostate carcinoma, and 15 Gleason pattern 4 prostate carcinoma. The second consisted of two replicate 1 mm cores of 65 visceral metastases (35-lymph node; 18-liver; 6-lung; 2-adrenal; 1-brain; 1-periaortic mass) and 120 bone metastases from 42 patients with CRPC as described previously [49 (link)]. Thirty-one of the 42 patients had matched bone and visceral metastases, 4 had only visceral metastases represented, and 7 had only bone metastases represented.