A Chromatin immunoprecipitation kit (Beyotime, China) was used for ChIP assay as previously described (13 (link)). E2F1 antibody and IgG rabbit antibody were obtained from Cell Signaling Technology (CST, USA) (Supplementary TableĀ 2). Reverse transcription PCR was performed to analyze the purified DNA. The primer sequences for DDX23 promoter are listed in Supplementary TableĀ 1.
Free full text: Click here