Cell Lysis and Western Blotting

Cells were lysed in lysis buffer supplemented with protease inhibitor cocktail (TargetMol, USA) at 4 °C for 20 min. The western blotting assay was performed as described previously [21 (link)].

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Wang J., Zhong Q., Zhang H., Liu S., Li S., Xia T., Xiao Z., Chen R., Ye Y., Liang F., Han P, & Huang X. (2022). Nogo-B promotes invasion and metastasis of nasopharyngeal carcinoma via RhoA-SRF-MRTFA pathway. Cell Death & Disease, 13(1), 76.

Publication 2022

protease inhibitor cocktail

Buffer Cells Protease inhibitor Western blotting assay

Corresponding Organization :

Other organizations : Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Sun Yat-sen University Cancer Center, The Seventh Affiliated Hospital of Sun Yat-sen University, Key Laboratory of Guangdong Province

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Variable analysis

independent variables

Lysis buffer supplemented with protease inhibitor cocktail

dependent variables

Protein expression levels (as measured by Western blotting)

control variables

Temperature (4 °C)
Lysis duration (20 min)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

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