Efficient Transduction and Selection Protocols
Corresponding Organization : University of Massachusetts Chan Medical School
Other organizations : Buck Institute for Research on Aging, Lawrence Berkeley National Laboratory
Protocol cited in 58 other protocols
Variable analysis
- Multiplicity of Infection (MOI) of viruses (between 0.5 and 1)
- Concentration of polybrene (6 µg/ml)
- Duration of virus and cell incubation (overnight)
- Timing of drug selection (48 h post-transduction)
- Concentration of selection drugs (see list in the input)
- Transduction efficiency (not explicitly mentioned)
- Cell types (WI38, HCA2, BJ, U2OS, HT1080, HeLa)
- Culture media (D-MEM)
- Volume of media per plate size (950 µl for 6-well, 5 ml for 10 cm, 11 ml for 15 cm)
- Timing of media change (next day after overnight incubation)
- Doxycycline concentration (1.0 µg/ml) and duration of induction (48 h for cDNA, 96 h for shRNA/miRNA)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
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