After anesthesia with ketamine and xylazine, an incision was made in the skin of each animal to reveal the skull, and after a careful cleaning of the aponeurosis, the lambda and bregma were detected. Subsequently, and in accordance with stereotactic coordinates ML = 1 mm, AP= −3.4 mm, and DV = 3 mm (Figure 1 A,B,C - according to the Atlas of Paxinos and Watson, 1998), the hippocampus was reached, where 3 µL solution of 5-OH-TRP (17 mg/mL) or 1% PBS at a rate of 1 µL/min were injected using a Hamilton syringe (Figure 1 D).
This was followed by the closure of the operative field and follow-up of the animal in a separate clean cage. The animal was subjected to behavioral tests 7 days after the stereotactic injection.
This was followed by the closure of the operative field and follow-up of the animal in a separate clean cage. The animal was subjected to behavioral tests 7 days after the stereotactic injection.
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