Reprogramming Human Fibroblasts to Pluripotent Stem Cells
Human fibroblasts from patients (Sainio et al., 2018 (link)) and healthy controls (Trokovic et al., 2015 (link)) were reprogrammed into pluripotent stem cells with overexpression of MYC, KLF4, SOX2, and OCT4 by the Biomedicum Stem Cell Center (University of Helsinki, Finland) as in Trokovic et al. (2015 (link)). Stem cells were grown in feeder-free conditions with Matrigel (Corning) and E8 with E8 supplement (Gibco) in normoxia at 37°C. At 90% confluency, cells were passaged with 0.5 mM EDTA (Invitrogen) in PBS.
Corresponding Organization : University of Helsinki
Other organizations :
University of Eastern Finland, Kuopio University Hospital, University of Gothenburg, University College London, Sahlgrenska University Hospital, UK Dementia Research Institute, Tampere University Hospital, Tampere University, Helsinki University Hospital
Reprogramming of human fibroblasts into pluripotent stem cells
control variables
Feeder-free culture conditions with Matrigel and E8 medium
Cell culture at 37°C in normoxia
Passage of cells at 90% confluency using 0.5 mM EDTA in PBS
controls
Healthy control human fibroblasts (Trokovic et al., 2015)
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