The presence of SARS-CoV-2 RNA in upper respiratory specimens were determined using one of two fully automated rtRT-PCR analyzers: Cobas 6800 (Roche Diagnostics, Basel, Switzerland) or Alinity m (Abbott, Chicago, IL, USA), as described previously [18 (link)].
The presence of total antibodies to the SARS-CoV-2 spike (S) protein receptor binding domain was determined using automated electrochemiluminescence immunoassay Elecsys Anti-SARS-CoV-2 S (Roche Diagnostics), as described previously [19 (link)]. Results were interpreted according to the manufacturer’s instructions.
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