Prostate Cancer Stem Cell Profiling

FACS analysis for prostate cancer stem cells was based on prior publications (1 (link),19 (link),21 (link)-23 (link)). Before labeling, the cells were blocked with normal mouse IgG in 1:100 dilution for 30 min and then incubated with fluorescein isothiocyanate (FITC) or phycoerythrin (PE)-labeled rat anti mouse Sca-1 (clone E13-161.7, Phamingen) (1/100-1/200), PE-labeled rat anti-mouseCD133 (1:10) (clone MB9-3G8, Miltenyl Biotec), PE/Cy5-labeled rat anti-human/mouse CD44 (1:200) (clone IM7, BioLegend, San Diego), PE/Cy5-labeled rat anti-human/mouse CD49f (1/10) and/or FITC-lebeled goat anti mouse Trop2 (FAB1122F, R&D) for 1 h. All experiments were conducted at 4°C. Cell sorting was performed on a FACS Calibur cell sorter (BD Biosciences). The data were analyzed with FlowJo software (Tree Star, Inc., Ashland, OR).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Ju X., Jiao X., Ertel A., Casimiro M.C., Di Sante G., Deng S., Li Z., Di Rocco A., Zhan T., Hawkins A., Stoyanova T., Ando S., Fatatis A., Lisanti M.P., Gomella L.G., Languino L.R, & Pestell R.G. (2016). SRC ONCOGENE INDUCES TROP2 PROTEOLYTIC ACTIVATION VIA CYCLIN D1. Cancer research, 76(22), 6723-6734.

Publication 2016

FACS Calibur cell sorter

Cancer stem cells Cd44 Cd49f Cell Clone Dilution Fluorescein Goat Human Isothiocyanate Mouse Phycoerythrin Prostate Sca 1 Tree

Corresponding Organization : Sidney Kimmel Cancer Center

Other organizations : University of California, Los Angeles, University of Calabria, Drexel University

Top 5 similar protocols

Variable analysis

independent variables

Incubation with fluorescein isothiocyanate (FITC) or phycoerythrin (PE)-labeled rat anti mouse Sca-1 (clone E13-161.7, Phamingen) (1/100-1/200)
Incubation with PE-labeled rat anti-mouseCD133 (1:10) (clone MB9-3G8, Miltenyl Biotec)
Incubation with PE/Cy5-labeled rat anti-human/mouse CD44 (1:200) (clone IM7, BioLegend, San Diego)
Incubation with PE/Cy5-labeled rat anti-human/mouse CD49f (1/10)
Incubation with FITC-lebeled goat anti mouse Trop2 (FAB1122F, R&D)

dependent variables

Cell sorting on a FACS Calibur cell sorter (BD Biosciences)

control variables

Blocking with normal mouse IgG in 1:100 dilution for 30 min
All experiments conducted at 4°C

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!