Metastatic-like tumors were established in C57BL/6 mice (Jackson Laboratory strain 00664), Rag−/− (Jackson Laboratory strain 002216), or IFNγ−/− (Jackson Laboratory strain 002287), as indicated, by intravenous injection of 3×105 B16-F10 (B16) melanoma cells. B16 cells were obtained from ATCC (CRL-6475), and tumors were established from cryo-preserved stocks that had been passaged less than two times. Tumor development was observed in 100% of injected mice.
Inducible melanomas were established by intradermal injection of 4-hydroxy-tamoxifen (10μl of a 20 μM solution in DMSO) in Tyr::CreER+/−; BrafCA/+; Ptenlox/lox mice (12 (link)) that had been backcrossed onto a B6 background, as described (13 (link)).
For all studies, animals were maintained in pathogen-free facilities at the Geisel School of Medicine, and all procedures were approved by the Dartmouth College Institutional Animal Care and Use Committee.
Inducible melanomas were established by intradermal injection of 4-hydroxy-tamoxifen (10μl of a 20 μM solution in DMSO) in Tyr::CreER+/−; BrafCA/+; Ptenlox/lox mice (12 (link)) that had been backcrossed onto a B6 background, as described (13 (link)).
For all studies, animals were maintained in pathogen-free facilities at the Geisel School of Medicine, and all procedures were approved by the Dartmouth College Institutional Animal Care and Use Committee.
