Characterization of FoxQ1-overexpressing breast cancer cells

Cell culture reagents including fetal bovine serum, cell culture media, phosphate-buffered saline (PBS), and antibiotic mixture were purchased from Life Technologies-Thermo Fisher Scientific (Waltham, MA). An antibody against FoxQ1 was from Santa Cruz Biotechnology (Dallas, TX). An antibody specific for detection of phospho-(Ser10) histone H3 was from Cell Signaling Technology (Danvers, MA). Anti-β-Actin antibody was from Sigma-Aldrich (St. Louis, MO). The MCF-7 cell line was purchased from the American Type Culture Collection and authenticated by us in 2015 and 2017. The SUM159 cell line was purchased from Asterand (Detroit, MI) and authenticated by us in 2015 and 2017. Details of stable transfection of MCF-7 and SUM159 cells with pCMV6 empty vector and the same vector encoding FoxQ1 and their culture conditions have been described by us previously.^{10 (link)} The HMLE cells stably transfected with FoxQ1 and EV cells were generously provided by Dr. Guojun Wu (Karmanos Cancer Institute, Departments of Oncology and Pathology, Wayne State University, Detroit, MI) and maintained as recommended by the provider.^{7 (link)}