All mice were maintained on B6 background, and all animal protocols were approved by the Weill Cornell Institutional Animal Care and Use Committee or the University of Alabama at Birmingham Animal Resources Program. Atp2a2flox (tm1c allele) mice were obtained from S. Kajimura (Ikeda et al., 2017 (link)). Genotyping was performed by PCR using Platinum Taq Polymerase (Invitrogen; 11304011) with the following primers: Atp2a2 Fwd: 5′-CAC​CTT​GTT​TAG​CCT​AGC​TTT​TTA​C-3′; and Atp2a2 Rev: 5′-GTT​GCA​CAC​TCT​TTC​TGT​CCT​G-3′. The PCR program is as follows: 94°C/2 min → [94°C/15 s → 58°C/30 s → 68°C/40 s] ×34 cycles → 68°C/3 min. The product size is 589 bp for WT and 700 bp for flox allele. To detect the knockout allele after crossing with Cre mice, a third primer (Atp2a2 3′LOXP.R1: 5′-ACT​GAT​GGC​GAG​CTC​AGA​CC-3′) was added together with the Fwd/Rev primer set. The product size for Cre-deleted allele is 200 bp.
Mb1-cre allele genotyping was performed using the above PCR conditions with the following primers: Mb1 Fwd: 5′-CTG​CGG​GTA​GAA​GGG​GGT​C-3′; Mb1 Rev: 5′-CCT​TGC​GAG​GTC​AGG​GAG​CC-3′; Mb1-Cre Fwd: 5′-ACC​TCT​GAT​GAA​GTC​AGG​AAG​AAC-3′; and Mb1-Cre Rev: 5′-GGA​GAT​GTC​CTT​CAC​TCT​GCT​TCT-3′ (Yen et al., 2019 (link)). The expected product size is 400 bp for WT and 500 bp for the Cre allele.