Plasma samples for analysis of axitinib and vascular endothelial growth factor ligand (VEGF) concentrations were collected on the same days as PET/CT scans: −3 to 0 days prior to dosing, after 12–14 days of dosing (week 2) and after 5–7 days of drug washout (week 3). VEGF concentrations were measured by a commercially available 96-well plate quantitative sandwich immunoassay (Quantikine® human VEGF, R&D Systems, Minneapolis, MN) according to manufacturer’s instructions. Axitinib plasma concentrations were measured by a validated LC/MS/MS as previously described [11 (link)].
Protocol detail
Quantification of Axitinib and VEGF Levels
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Axitinib
Drug
Human vascular endothelial growth factor
Immunoassay
Ligand
Ms ms
Pet ct scans
Plasma
Vascular endothelial growth factor
Corresponding Organization : University of Wisconsin Carbone Cancer Center
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Variable analysis
independent variables
- Days prior to dosing (-3 to 0 days)
- Week 2 (12-14 days of dosing)
- Week 3 (5-7 days of drug washout)
- Axitinib plasma concentrations
- VEGF concentrations
- PET/CT scans conducted on the same days as plasma sample collection
- Positive control: Not specified
- Negative control: Not specified
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