Sodium alginate solution was made by dissolving 1% (w/v) Sodium alginate (Sigma-Aldrich, UK) in deionized (DI) water. calcium chloride solution was prepared by dissolving 4% (w/v) calcium chloride (CaCl2) (Sigma-Aldrich, St. Louis, MO) in DI water. For constructing fibrin scaffolds, fibrinogen (6 mg/ml) (Sigma-Aldrich) and thrombin (2.4 U/ml) (Sigma-Aldrich) were prepared for microvalve bioprinting. For bioprinting of electrocytes, agarose (A20070-100, Research Products International, IL) was dissolved in DI water to obtain a solution of 1% (w/v) agarose at 90°C. COL I was extracted from rat tails according to a published protocol (54 (link)). GelMA was synthesized according to an established protocol (55 (link)). Detailed preparation methods of both materials can be found in the Supplementary Materials.
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Hydrogel Scaffold Preparation Protocols
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Corresponding Organization :
Other organizations : Pennsylvania State University, Kyung Hee University Dental Hospital
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Variable analysis
independent variables
- Sodium alginate concentration (1% w/v)
- Calcium chloride concentration (4% w/v)
- Fibrinogen concentration (6 mg/ml)
- Thrombin concentration (2.4 U/ml)
- Agarose concentration (1% w/v)
- Not explicitly mentioned
- Deionized water used as solvent
- Temperature maintained at 90°C for agarose solution
- Positive control: Not mentioned
- Negative control: Not mentioned
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