Immunofluorescence and RNA FISH Protocol
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Centre National de la Recherche Scientifique
Other organizations : Instituto de Biologia Experimental e Tecnológica, Universidade Nova de Lisboa, Université de Lorraine, Inserm
Variable analysis
- None explicitly mentioned
- Localization and expression of U3 and U85 snoRNAs
- Cell culture conditions (growth on coverslips)
- Cell fixation and permeabilization methods (4% paraformaldehyde, 0.1% Triton X-100 in PBS)
- Primary and secondary antibody incubation conditions (3% BSA, room temperature)
- Washing steps (3 times in PBS)
- FISH hybridization and washing conditions (10% formamide in 2XSSC, PBS)
- Mounting medium (Vectashield with DAPI)
- Microscopy setup (upright epifluorescence microscope, 63x oil objective)
- None explicitly mentioned
- None explicitly mentioned
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!