ON-TARGETplus SMARTpool control siRNA and PAK6 siRNA were obtained from Dharmacon (Lafayette, CO). The H358-smoke, H1299, H1650 and H1703 cells were transfected with RNAiMAX (Invitrogen, Grand Island, NY) following manufacturer's instructions. Transfection was carried out as previously described [65 (link), 71 (link)]. Cells were subjected to invasion assays and colony formation assays 48 hours post-transfection, unless otherwise stated.
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