Adipose tissue immunostaining protocol

Adipose tissues were harvested from perfused (4% paraformaldehyde) mice. Paraffin embedding and tissue sectioning was performed by the Molecular Pathology Core Facility at UTSW. Indirect immunofluorescence was performed as previously described (Vishvanath et al., 2016 (link)). Antibodies used for immunofluorescence include: anti-GFP 1:700 (Abcam ab13970), anti-perilipin 1:1500 (Fitzgerald 20R-PP004), anti-chicken Alexa 488 1:200 (Invitrogen), and anti-guinea pig Alexa 647 1:200 (Invitrogen). Hematoxylin and eosin staining was performed according to manufacturer’s instructions.

Free full text: Click here

Hepler C., Shan B., Zhang Q., Henry G.H., Shao M., Vishvanath L., Ghaben A.L., Mobley A.B., Strand D., Hon G.C, & Gupta R.K. (2018). Identification of functionally distinct fibro-inflammatory and adipogenic stromal subpopulations in visceral adipose tissue of adult mice. eLife, 7, e39636.

Publication 2018

20R-PP004 anti-chicken Alexa 488 anti-guinea pig Alexa 647

Adipose tissues Antibodies Chicken Eosin Guinea pig Hematoxylin Immunofluorescence Indirect immunofluorescence Mice Paraformaldehyde Perilipin 1 Tissue

Corresponding Organization :

Other organizations : The University of Texas Southwestern Medical Center

Top 5 similar protocols

Variable analysis

independent variables

Perfusion with 4% paraformaldehyde

dependent variables

Adipose tissue harvested from mice
Immunofluorescence staining for GFP, perilipin, Alexa 488, and Alexa 647
Hematoxylin and eosin staining

control variables

Paraffin embedding and tissue sectioning performed by the Molecular Pathology Core Facility at UTSW

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!