Comprehensive Antibody Catalogue for Muscle and Calcium Signaling

Primary antibodies against PLN (2D12), phosphorylated-NFATc1 (PA5-38301), total NFATc1 (MA3-024), SERCA2a (MA3-919), ryanodine receptor 1 (RyR, MA3-925), dihydropyridine receptor α1 subunit (DHPR, MA3-920), and calsequesterin I and II (CSQ, MA3-913) were obtained from Pierce Antibodies. The primary antibody directed against SLN was generated by Lampire Biological Laboratories (PA, USA) [28 (link)]. LC3B (2775) and p62 (GP62-C) antibodies were obtained from Cell Signaling Technology (MA, USA) and Progen Biotechnik (Heidelberg, Germany), respectively. The primary antibody against actin (A2066) and stabilin-2 (orb158499) were obtained from Sigma Aldrich (MO, USA), and Biorbyt (CA, USA), respectively. SERCA1a antibody (A52) was a kind gift from Dr. David MacLennan (University of Toronto) [29 (link)]. The primary antibodies against MHCI (BA-F8), MHCIIa (SC-71), and MHCIIb (BF-F3) were obtained from Developmental Studies Hybridoma Bank (IA, USA). Secondary antibodies for western blotting, goat anti-mouse IgG (peroxidase conjugated) and goat anti-rabbit IgG (peroxidase conjugated) were obtained from Santa Cruz Biotechnology (TX, USA). Secondary antibodies for immunofluorescence staining, Alexa Fluor 350 anti-mouse IgG_2b, Alexa Fluor 488 anti-mouse IgG₁, and Alexa Fluor 555 anti-mouse IgM, were obtained from Molecular Probes (OR, USA).

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Fajardo V.A., Gamu D., Mitchell A., Bloemberg D., Bombardier E., Chambers P.J., Bellissimo C., Quadrilatero J, & Tupling A.R. (2017). Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice. PLoS ONE, 12(3), e0173708.

Publication 2017

LC3B (2775) BA-F8), SC-71), Alexa Fluor 350 anti-mouse IgG2b Alexa Fluor 488 anti-mouse IgG1 Alexa Fluor 555 anti-mouse IgM

Actin Alexa fluor 350 Alexa fluor 488 Alexa fluor 555 Anti igg Anti igm Antibodies Antibody Biological Dihydropyridine receptor α1 subunit Goat Hybridoma Igg1 Igg2b Immunofluorescence Molecular probes Mouse Peroxidase Progen Rabbit Ryanodine receptor 1

Corresponding Organization : University of Waterloo

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Variable analysis

independent variables

Primary antibodies against PLN (2D12), phosphorylated-NFATc1 (PA5-38301), total NFATc1 (MA3-024), SERCA2a (MA3-919), ryanodine receptor 1 (RyR, MA3-925), dihydropyridine receptor α1 subunit (DHPR, MA3-920), and calsequesterin I and II (CSQ, MA3-913) obtained from Pierce Antibodies
Primary antibody directed against SLN generated by Lampire Biological Laboratories (PA, USA)
LC3B (2775) and p62 (GP62-C) antibodies obtained from Cell Signaling Technology (MA, USA) and Progen Biotechnik (Heidelberg, Germany), respectively
Primary antibody against actin (A2066) and stabilin-2 (orb158499) obtained from Sigma Aldrich (MO, USA), and Biorbyt (CA, USA), respectively
SERCA1a antibody (A52) obtained from Dr. David MacLennan (University of Toronto)
Primary antibodies against MHCI (BA-F8), MHCIIa (SC-71), and MHCIIb (BF-F3) obtained from Developmental Studies Hybridoma Bank (IA, USA)

dependent variables

Not explicitly mentioned

control variables

Goat anti-mouse IgG (peroxidase conjugated) and goat anti-rabbit IgG (peroxidase conjugated) secondary antibodies obtained from Santa Cruz Biotechnology (TX, USA)
Alexa Fluor 350 anti-mouse IgG2b, Alexa Fluor 488 anti-mouse IgG1, and Alexa Fluor 555 anti-mouse IgM secondary antibodies obtained from Molecular Probes (OR, USA)

positive controls

Not specified

negative controls

Not specified

Annotations

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