Protocol detail

Immunoblot and Immunohistochemical Analysis

Find Similar Protocols

Immunoblot and immunohistochemical analyses were performed according to the method described previously [18 (link), 34 (link)]. Rabbit polyclonal antibodies against DTX3L/Dtx3l (Santa Cruz), phosphorylated threonine 202 in ERK1 and phosphorylated tyrosine 204 in ERK2 (Cell Signaling), phosphorylated tyrosine 397 in FAK (Invitrogen), phosphorylated MEK1/2 (Cell Signaling), PI3K and phosphorylated PI3K (Cell Signaling); rabbit monoclonal antibodies against Akt and phosphorylated Akt (Cell Signaling); and mouse monoclonal antibodies against alpha-TUBULIN (SIGMA), MEK1/2 (Cell Signaling), ERK1/2 (Cell Signaling) and FAK (Millipore) were used as first antibodies. Immunohistochemistry was performed according to the method previously described [34 (link)].

Free full text: Click here

Thang N.D., Yajima I., Kumasaka M.Y., Iida M., Suzuki T, & Kato M. (2015). Deltex-3-like (DTX3L) stimulates metastasis of melanoma through FAK/PI3K/AKT but not MEK/ERK pathway. Oncotarget, 6(16), 14290-14299.

Publication 2015

phosphorylated MEK1/2 phosphorylated PI3K phosphorylated Akt MEK1/2 ERK1/2

Alpha tubulin Antibodies Erk1 Erk2 Immunoblot Immunohistochemistry Mek1 Monoclonal antibodies Mouse Pi3k Rabbit Threonine Tyrosine

Top 5 similar protocols

Variable analysis

independent variables

Rabbit polyclonal antibodies against DTX3L/Dtx3l, phosphorylated threonine 202 in ERK1, phosphorylated tyrosine 204 in ERK2, phosphorylated tyrosine 397 in FAK, phosphorylated MEK1/2, PI3K and phosphorylated PI3K
Rabbit monoclonal antibodies against Akt and phosphorylated Akt
Mouse monoclonal antibodies against alpha-TUBULIN, MEK1/2, ERK1/2 and FAK

dependent variables

Immunoblot and immunohistochemical analyses

control variables

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!