Folin-Ciocalteu reagent, aluminum chloride, ferric chloride, hydrogen peroxide, Potassium ferricyanide, sodium phosphate (monobasic and dibasic), sodium carbonate, 2,4,6-tripyridyl-s-triazine (TPTZ), ferrozine, ferrous chloride, ammonium molybdate, sodium phosphate and sulphuric acid, 1,10-phenanthroline, ascorbic acid, tannic acid, rutin, acetone, ethanol and methanol (HPLC grade) were procured from HiMedia Chemical Co. Mumbai, (India). All the solvents used during the study were of AR grade.