SH-SY5Y neuroblastoma cell line (ATCC number CRL-2266) was maintained in a 1:1 mix of Minimal Essential Medium Eagle (Sigma) and Nutrient Mixture F-12 Ham (Sigma), supplemented with 10% foetal bovine serum (Sigma), 1% penicillin/streptomycin (100 U/ml, 100 mg/ml; Sigma), 1% (v/v) 200 mM l -glutamine (Sigma), and 1% (v/v) 100 mM sodium pyruvate (Sigma). Cells were incubated at 37 °C with 5% CO2.
Stock solutions were made for amphetamine, cocaine hydrochloride, lithium chloride, and valproic acid sodium salt (Sigma) using sterile filtered dH2O, and diluted as appropriate in SH-SY5Y media. Cells were cultivated for 24 h, before a 1 h incubation with either: vehicle control (sterile filtered dH2O), 10 μM amphetamine (Jones and Kauer, 1999 , Shyu et al., 2004 (link)), 10 μM cocaine (Warburton et al., 2015c (link)), 1 mM lithium (Hing et al., 2012 (link), Roberts et al., 2007 ) or 5 mM sodium valproate (Pan et al., 2005 (link), Phiel et al., 2001 (link), Zhang et al., 2003 ). For each drug treatment, n = 4. Cells were harvested directly after the 1 h drug treatment for RNA extraction.
Stock solutions were made for amphetamine, cocaine hydrochloride, lithium chloride, and valproic acid sodium salt (Sigma) using sterile filtered dH2O, and diluted as appropriate in SH-SY5Y media. Cells were cultivated for 24 h, before a 1 h incubation with either: vehicle control (sterile filtered dH2O), 10 μM amphetamine (Jones and Kauer, 1999 , Shyu et al., 2004 (link)), 10 μM cocaine (Warburton et al., 2015c (link)), 1 mM lithium (Hing et al., 2012 (link), Roberts et al., 2007 ) or 5 mM sodium valproate (Pan et al., 2005 (link), Phiel et al., 2001 (link), Zhang et al., 2003 ). For each drug treatment, n = 4. Cells were harvested directly after the 1 h drug treatment for RNA extraction.
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