Total RNA was extracted from ovaries and uterine tissue using Trizol reagent (Invivogen, Shanghai, China) following the manufacturer's protocol (13 (link)). Relative mRNA expression levels (Table 1 ) were determined using the Takara PrimeScriptTM RT reagent kit with gDNA Eraser (Takara, Dalian, China), and the cDNAs were run on a Bio-Rad CFX-96 thermocycler (Bio-Rad, CA, USA). The primers used and the product size are shown in Table 1 . The relative expression levels were calculated by the 2−ΔΔCT method and the cytoskeletal protein, β- actin, was included as endogenous control to normalize the data.
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