SARS-CoV-2 Beta Variant Infection in Hamsters

The hamster infection model of SARS-CoV-2 has been described before^{16 (link),20 (link)}. Female Syrian hamsters (Mesocricetus auratus) were purchased from Janvier Laboratories and kept per two in individually ventilated isolator cages (IsoCage N Bio-containment System, Tecniplast) at 21 °C, 55% humidity and 12:12 day/night cycles. Housing conditions and experimental procedures were approved by the ethics committee of animal experimentation of KU Leuven (license P065-2020). For infection, female hamsters of 6–8 weeks old were anesthetized with ketamine/xylazine/atropine and inoculated intranasally with 50 µL containing 10⁴ TCID₅₀ of SARS-CoV-2 Beta variant B.1.351 (day 0). On day 4 pi, animals were euthanized for the sampling of the lungs and further analysis by i.p. injection of 500 μl Dolethal (200 mg/ml sodium pentobarbital, Vétoquinol SA). All caretakers and technicians were blinded to group allocation in the animal facility.

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Abdelnabi R., Foo C.S., Jochmans D., Vangeel L., De Jonghe S., Augustijns P., Mols R., Weynand B., Wattanakul T., Hoglund R.M., Tarning J., Mowbray C.E., Sjö P., Escudié F., Scandale I., Chatelain E, & Neyts J. (2022). The oral protease inhibitor (PF-07321332) protects Syrian hamsters against infection with SARS-CoV-2 variants of concern. Nature Communications, 13, 719.

Publication 2022

IsoCage N Bio-containment System

Animal Animal ethics committee Atropine Female Hamsters Humidity Infection Ketamine Lungs Mesocricetus auratus Sars cov 2 b 1 351 variant Sars cov 2 infection Sodium pentobarbital Xylazine

Corresponding Organization :

Other organizations : Rega Institute for Medical Research, KU Leuven, Mahidol Oxford Tropical Medicine Research Unit, Mahidol University, Drugs for Neglected Diseases Initiative, Global Virus Network

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Variable analysis

independent variables

SARS-CoV-2 Beta variant B.1.351 (10^4 TCID50)

dependent variables

Lung samples taken on day 4 post-infection for further analysis

control variables

Female Syrian hamsters (Mesocricetus auratus) aged 6-8 weeks
Kept in individually ventilated isolator cages at 21°C, 55% humidity, and 12:12 day/night cycles
Housing conditions and experimental procedures approved by the ethics committee

positive controls

None specified

negative controls

None specified

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