Hematological Biomarkers and Cognitive Decline

Participants were asked to fast after 8:00 p.m., the evening before their clinic exam, and considered to be fasting after a minimum of a 10-h fast. Blood was drawn from participants in a supine position, using standard venipuncture technique, typically between 7:00 a.m. and 9:00 a.m. Hematology testing was performed using whole blood [Tyco Monoject, 15% ethylenediaminetetraacetic acid, EDTA, (K3)]. A CBC with differential was performed on EDTA whole blood using a Beckman Coulter HmX Hematology Analyzer as described previously (Sloan et al., 2015 (link)). Blood collection was performed following the exact same procedures in every subject. Hematology testing included estimation of intra-assay CV in ∼10% samples run in duplicate. The NLR was defined as the absolute neutrophil count by the absolute lymphocyte count from the CBC panel. Assessment of cognitive decline and dementia was performed by a panel blinded to the CBC/NLR results.

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Ramos-Cejudo J., Johnson A.D., Beiser A., Seshadri S., Salinas J., Berger J.S., Fillmore N.R., Do N., Zheng C., Kovbasyuk Z., Ardekani B.A., Bubu O.M., Parekh A., Convit A., Betensky R.A., Wisniewski T.M, & Osorio R.S. (2021). The Neutrophil to Lymphocyte Ratio Is Associated With the Risk of Subsequent Dementia in the Framingham Heart Study. Frontiers in Aging Neuroscience, 13, 773984.

Publication 2021

HmX Hematology Analyzer

Assay Blood Cognitive decline Dementia Edta Lymphocyte count Neutrophil Venipuncture

Corresponding Organization : Nathan Kline Institute for Psychiatric Research

Other organizations : National Heart Lung and Blood Institute, Framingham Heart Study, Boston University, The University of Texas Health Science Center at San Antonio, Center for Neuro-Oncology, Harvard University, Icahn School of Medicine at Mount Sinai

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Variable analysis

independent variables

Fasting status: Participants were asked to fast after 8:00 p.m. the evening before their clinic exam

dependent variables

Hematology parameters: Whole blood was analyzed for a complete blood count (CBC) with differential using a Beckman Coulter HmX Hematology Analyzer
Neutrophil-to-lymphocyte ratio (NLR): Calculated from the CBC panel as the absolute neutrophil count divided by the absolute lymphocyte count
Cognitive decline and dementia: Assessed by a panel blinded to the CBC/NLR results

control variables

Fasting duration: Participants were considered to be fasting after a minimum of a 10-h fast
Blood collection: Performed in a supine position, using standard venipuncture technique, typically between 7:00 a.m. and 9:00 a.m.
Blood processing: Hematology testing was performed using whole blood collected in EDTA tubes
Intra-assay precision: Estimated by running approximately 10% of the samples in duplicate

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