Two-months-old no carrier male mice (control mice) and 2- and 6-month-old male B6SJL-Tg(APPSwFlLon, PSEN1 ∗ M146L ∗ L286V)6799Vas/Mmjax (5xFAD) mice were used for a sleep fragmentation protocol. Experimental procedures involving the use of live animals have been carried out by the guidelines established by the European Community Directive 86/609/EEC (November 24, 1986), Italian Ministry of Health and the University of Turin institutional guidelines on animal welfare (law 116/92 on Care and Protection of living animals undergoing experimental or other scientific procedures; authorization number: 470/2021-PR). Moreover, the Ethical Committee of the University of Turin approved this type of study. The animals were maintained under 12-h light/dark cycles and were provided with water and food ‘‘ad libitum’’ (standard mouse chow 4RF25-GLP, Mucedola srl, Settimo Milanese, Italy). Specifically, all the procedures were carried out in order to minimize the pain and distress in the animals and we used the fewest number of animals required to obtain statistically significant data.
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