Because caliper measurement is not feasible for measuring peritoneal tumor burden, we used bioluminescent imaging as a surrogate for tumor burden, as previously described.49 50 (link) All animals were imaged with bioluminescence for luciferase activity in the peritoneum to identify peritoneal tumor implants and growth after IP SNU-16-ffluc, and the tumor burden was quantified once a week after treatment. D-luciferin solution was prepared by dissolving 1 g of IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (PerkinElmer, catalog#122799-5, Waltham, Massachusetts, USA) in 35 mL of PBS at 28.5 mg/mL concentration. IP delivery (200 µL/mouse) was performed in all groups, and the mice were imaged using Lago X optical imaging system (Spectral Instruments Imaging, Tucson, Arizona, USA). Bioluminescence imaging was analyzed using Aura V.64 software and presented as photons/second for regions of interest.41 (link)
Protocol detail
Peritoneal Tumor Burden Quantification
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After treatment
Animals
Delivery
Luciferase
Luciferin
Mice
Peritoneal tumor
Peritoneum
Potassium
Salt
Tumor burden
Corresponding Organization : City Of Hope National Medical Center
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Variable analysis
independent variables
- IP delivery of D-luciferin solution (200 µL/mouse)
- Bioluminescence imaging for luciferase activity in the peritoneum to identify peritoneal tumor implants and growth after IP SNU-16-ffluc
- Tumor burden quantified once a week after treatment
- Mice were imaged using Lago X optical imaging system (Spectral Instruments Imaging, Tucson, Arizona, USA)
- Bioluminescence imaging was analyzed using Aura V.64 software
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