Synthesis and Characterization of PCB Metabolites

Human serum albumin (fatty acid and globulin free, ≥99% pure), phenylbutazone, and 5-dimethylamino-1-naphthalenesulfonamide (dansylamide; DNSA) were purchased from Sigma-Aldrich (St. Louis, MO). Flufenamic acid was purchased from Fluka Analytical (Buchs, Switzerland), and dansyl-L-proline (DP) was purchased from TCI (Cambridge, MA). The synthesis and characterization of 4 OH PCB 52 (2,2’,5,5’-tetrachlorobiphenyl-4-ol) and 4 PCB 52 sulfate (sulfuric acid mono-(2,2’,5,5’-tetrachlorobiphenyl-4-yl) ester, ammonium salt) are reported in the Supporting Information. All other PCB metabolites were prepared and characterized in the synthesis core of the Iowa Superfund Research Program as previously described.^{43 (link)–45 (link)}