Salvianolic Acid B Ameliorates Fibrosis

Thirty female mice (8 weeks old, C57BL/6J) were randomly divided into five groups (n = 6). To remedy fibrosis, injured mice were administered 100 μL intraperitoneal injections of either salvianolic acid B (Sigma) at 50, 100, or 200 µg/mL in PBS, or PBS alone, every day until 8 weeks after surgery. The SAB doses chosen were based on the literature [45 (link)]. Care was taken to avoid direct exposure of SAB to light. All functional analyses were performed at 2 days and weekly for 4 weeks post-infection. Hindlimb locomotor function was assessed using the Basso Mouse Scale (BMS) [46 (link)]. Briefly, patterns of hind limb movement, plantar stepping, and paw positions were observed in each animal. All mice were habituated to the BMS open-field arena in 10-min sessions every day for 1 week [15 (link)]. The locomotor score was assessed by two blinded independent observers.