Quantifying Protein Expression via Western Blotting

Western blotting (WB) was performed according to a standard method as described previously [25 (link)]. The following primary antibodies were used: anti-Trop2 (R&D Systems, Minneapolis, MN), anti-ERK1/2 and anti-phospho-ERK1/2 (Cell Signaling Technology, Beverly, MA), anti-p38, anti-phospho-p38, anti-JNK, and anti-phospho-JNK (Abcam, Cambridge, MA), and anti-α-tubulin (Sigma-Aldrich, St. Louis, MO).

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Guan H., Guo Z., Liang W., Li H., Wei G., Xu L., Xiao H, & Li Y. (2017). Trop2 enhances invasion of thyroid cancer by inducing MMP2 through ERK and JNK pathways. BMC Cancer, 17, 486.

Publication 2017

anti-Trop2 anti-ERK1/2 anti-phospho-ERK1/2 anti-p38 anti-phospho-p38 anti-JNK anti-phospho-JNK anti-α-tubulin

Antibodies Erk1 α tubulin

Corresponding Organization :

Other organizations : Sun Yat-sen University, The First Affiliated Hospital, Sun Yat-sen University

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Protocol cited in 2 other protocols

Variable analysis

independent variables

Primary antibodies used: anti-Trop2, anti-ERK1/2, anti-phospho-ERK1/2, anti-p38, anti-phospho-p38, anti-JNK, and anti-phospho-JNK, and anti-α-tubulin

dependent variables

Protein expression and phosphorylation levels of Trop2, ERK1/2, p38, and JNK

control variables

Western blotting (WB) was performed according to a standard method as described previously [25 (link)]

controls

No positive or negative controls were explicitly mentioned

Annotations

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