Biosorption Capabilities of Bacterial Isolates

The biosorption capability of bacterial isolates was assayed as above; the biosorption of metal ions was measured by Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES optima 8300, Perkin Elmer, Massachusetts, USA). The ICP-OES was calibrated with standard working metal solutions and blank as above to set limits of detection (10–1000 ppm). The emission lines used for the analysis were 327.393 nm, 228.802 nm, and 340.458 nm for copper, cadmium, and lead, respectively, under Argon plasma with the concentric nebulizer. The residual metal concentration was deduced from internal standard curve produced from standardisation before running the samples and culture free control [21 (link)].

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Syed S, & Chinthala P. (2015). Heavy Metal Detoxification by Different Bacillus Species Isolated from Solar Salterns. Scientifica, 2015, 319760.

Publication 2015

ICP-OES optima 8300

Argon Bacterial Cadmium Copper Emission Ions Metal Nebulizer Plasma Spectroscopy

Corresponding Organization :

Other organizations : Sri Venkateswara University

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Variable analysis

independent variables

Bacterial isolates

dependent variables

Biosorption capability of bacterial isolates
Biosorption of metal ions (copper, cadmium, and lead)

control variables

Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) parameters (emission lines, Argon plasma, concentric nebulizer)
Calibration of ICP-OES with standard working metal solutions and blank to set limits of detection (10–1000 ppm)
Culture free control

controls

Positive control: Standard working metal solutions
Negative control: Blank

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