Antibody Characterization for Protein Studies

The following antibodies were used in this study: anti-Sec61α (1:5000), anti-Sec61β (1:5000), anti-TRAPα (1:5000), anti-SRα (1:5000), anti-FLAG (1:1000), and anti-PrP-A antibodies (1:5000), which have been previously described [7 (link),10 (link),18 (link)]. Anti-clanexin (1:1000), anti-BiP (1:1000), and anti-calreticulin antibodies (1:1000) were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-PDI (1:5000) and anti-HA-conjugated magnetic beads were obtained from Thermo Fisher Scientific (Waltham, MA, USA). A PrP-specific 3F4 antibody (1:10,000) was purchased from BioLegend (San Diego, CA, USA). Anti-FLAG (M2)-conjugated magnetic beads, FLAG peptide, DTT, thapsigargin (Tg), and all chemicals for biochemical analyses were purchased from Sigma-Aldrich Korea (Seoul, Korea).