Cell lysates were prepared using the radioimmunoprecipitation (RIPA) buffer (Nacalai Tesque, Kyoto, Japan) or M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) supplemented with the protease inhibitor cocktail (Nacalai Tesque). Western blot analysis was performed as described12 (link). Images were obtained with the GE Amersham Imager 600 (GE Healthcare, Milwaukee, WI, USA) or FUSION SOLO.7S.EDGE (Vilber-Lourmat, Marne la Vallée, France).
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