Biochemical Analysis of Serum Samples

Blood samples, collected at the end of the treatments, were centrifuged at 1700 g for 10 min at room temperature and serum was stored at −20°C until use. By using commercial reagents (Siemens Healthcare Diagnostics s.r.l., Milan, Italy) and an automated biochemistry analyzer (Dimension EXL, Siemens Healthcare Diagnostics s.r.l.) the levels of basal glucose, triglycerides, total cholesterol, low density lipoproteins cholesterol (LDL), high density lipoproteins cholesterol (HDL), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were determined. The concentration of insulin and leptin was measured using ELISA kits (Rat/Mouse Insulin ELISA Kit; Mouse Leptin ELISA Kit, EMD Millipore Corporation, Darmstadt, Germany) according to the manufacturers' instructions. Approximate insulin resistance (IR) was calculated using the homeostasis model assessment (HOMA)-IR using the following formula: [glucose (mmol/L) × insulin (μU/mL)]/22.5 [28 (link)].

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Lepore S.M., Morittu V.M., Celano M., Trimboli F., Oliverio M., Procopio A., Di Loreto C., Damante G., Britti D., Bulotta S, & Russo D. (2015). Oral Administration of Oleuropein and Its Semisynthetic Peracetylated Derivative Prevents Hepatic Steatosis, Hyperinsulinemia, and Weight Gain in Mice Fed with High Fat Cafeteria Diet. International Journal of Endocrinology, 2015, 431453.

Publication 2015

Dimension EXL Rat/Mouse Insulin ELISA Kit Mouse Leptin ELISA Kit

Alanine aminotransferase Aspartate aminotransferase Blood Cholesterol Diagnostics Elisa Glucose High density lipoproteins cholesterol Homeostasis Insulin Insulin resistance Leptin Low density lipoproteins cholesterol Mouse Serum Triglycerides

Corresponding Organization : Magna Graecia University

Other organizations : University of Udine

Top 5 similar protocols

Variable analysis

independent variables

Treatment

dependent variables

Basal glucose
Triglycerides
Total cholesterol
Low density lipoproteins cholesterol (LDL)
High density lipoproteins cholesterol (HDL)
Alanine aminotransferase (ALT)
Aspartate aminotransferase (AST)
Insulin
Leptin
Insulin resistance (IR) calculated using HOMA-IR

control variables

Centrifugation at 1700 g for 10 min at room temperature
Storage of serum at -20°C until use
Use of commercial reagents and automated biochemistry analyzer
Use of ELISA kits to measure insulin and leptin

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