Amplification primers were designed to cover the exonic regions of five genes, GRIK1 (GeneID:2897), GRIK2 (GeneID:2898), GRIK3 (GeneID:2899), GRIK4 (GeneID:2900), and GRIK5 (GeneID:2901), with the Ion AmpliSeq™ Designer software (Thermo Fisher Scientific Inc., Waltham, MA, USA). Ion semiconductor sequencing, PCR reactions, and fluorescence-based cycle sequencing were performed following previously described methods [41 (link)].
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