Cell viability was analyzed 48 h and 72 h after TKI treatment by crystal violet staining (C6158, Sigma, France), as previously described [42 (link)]. Plated cells were washed with 1× PBS and incubated min with 0.1% crystal violet solution for 20 min. After several washes, 10% acetic acid solution was added to lyse-stained cells. Absorbance was measured at 595 nm using an Infinite 200 Pro Microplate Luminometer (Tecan Trading AG).
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