Real-Time Cell Adhesion Analysis

Real time cell index analysis was performed to measure focal adhesion of live cells using xCELLigence real time cell analyser (RTCA; ACEA Biosciences) [30 (link)]. The xCELLigence biosensor measured cellular adhesion and the cell index (unitless) was determined by the xCELLigence software (version 1.2.1). Cells were seeded onto custom RTCA E16 plates (ACEA Biosciences), coated with high-density gold arrays for measuring electric impedance. The cells were incubated for 24 h to attain a stable cell index followed by treatment with binase or combination of binase and IFNα2b. Cell index measurements were recorded every 15 min during the course of cell proliferation.

Free full text: Click here

Mitkevich V.A., Burnysheva K.M., Petrushanko I.Y., Adzhubei A.A., Schulga A.A., Chumakov P.M, & Makarov A.A. (2017). Binase treatment increases interferon sensitivity and apoptosis in SiHa cervical carcinoma cells by downregulating E6 and E7 human papilloma virus oncoproteins. Oncotarget, 8(42), 72666-72675.

Publication 2017

xCELLigence real time cell analyser (RTCA;

Binase Biosensor Cell Cell proliferation Cellular adhesion Electric impedance Gold Ifnα2b Rtca

Corresponding Organization :

Other organizations : Engelhardt Institute of Molecular Biology, Institute of Bioorganic Chemistry

Top 5 similar protocols

Variable analysis

independent variables

Binase
Combination of binase and IFNα2b

dependent variables

Cellular adhesion
Cell index (unitless)

control variables

Incubation time (24 h)
Cell seeding onto custom RTCA E16 plates coated with high-density gold arrays

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!