Effect of SNG on the MMP of MM cells was determined using BD MitoScreen (JC-1) assay kit as described earlier (45 (link)). After incubation with SNG, cells in 1x assay buffer were incubated with JC-1 stain for 15 min at 37°C, washed and then analyzed by Flow Cytometry, to quantify the reduction in red fluorescence.
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