ALDH activity was analyzed as previously described15 (link). Shortly, cells were treated for 4 days with WF, RT-WF and WF + RIBE fluids, detached with Accutase (BioWest, France) and subjected to ALDEFLUOR Assay based on manufacturer protocol. For each sample negative controls were incubated under the same conditions with diethylaminobenzaldehyde (DEAB).
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